January 10, 2017

Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass

Biotechnology for Biofuels | 2017 | DOI: https://doi.org/10.1186/s13068-016-0697-5

Suresh Poudel, Richard J. Giannone, Miguel Rodriguez Jr., Babu Raman, Madhavi Z. Martin, Nancy L. Engle, Jonathan R. Mielenz, Intawat Nookaew, Steven D. Brown, Timothy J. Tschaplinski, David Ussery, Robert L. Hettich


Clostridium thermocellum is capable of solubilizing and converting lignocellulosic biomass into ethanol. Although much of the work-to-date has centered on characterizing this microbe’s growth on model cellulosic substrates, such as cellobiose, Avicel, or filter paper, it is vitally important to understand its metabolism on more complex, lignocellulosic substrates to identify relevant industrial bottlenecks that could undermine efficient biofuel production. To this end, we have examined a time course progression of C. thermocellum grown on switchgrass to assess the metabolic and protein changes that occur during the conversion of plant biomass to ethanol.

Read more: https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0697-5